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Production and Isolation of Azaspiracid-1 and -2 from Azadinium spinosum Culture in Pilot Scale Photobioreactors ArchiMer
Jauffrais, Thierry; Kilcoyne, Jane; Sechet, Veronique; Herrenknecht, Christine; Truquet, Philippe; Herve, Fabienne; Berard, Jean-baptiste; Nulty, Ciara; Taylor, Sarah; Tillmann, Urban; Miles, Christopher O.; Hess, Philipp.
Azaspiracid (AZA) poisoning has been reported following consumption of contaminated shellfish, and is of human health concern. Hence, it is important to have sustainable amounts of the causative toxins available for toxicological studies and for instrument calibration in monitoring programs, without having to rely on natural toxin events. Continuous pilot scale culturing was carried out to evaluate the feasibility of AZA production using Azadinium spinosum cultures. Algae were harvested using tangential flow filtration or continuous centrifugation. AZAs were extracted using solid phase extraction (SPE) procedures, and subsequently purified. When coupling two stirred photobioreactors in series, cell concentrations reached 190,000 and 210,000 cell·mL−1 at...
Tipo: Text Palavras-chave: Solid phase extraction; Photobioreactor; Chemostat; Dinoflagellate; Micro-algae; LC-MS/MS; Tangential flow filtration; Azaspiracid; HP-20.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00088/19875/17526.pdf
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Results from Verification of Reference Irradiance and Radiance Sources Laboratory Calibration Experiment Campaign ArchiMer
Białek, Agnieszka; Goodman, Teresa; Woolliams, Emma; Brachmann, Johannes F. S.; Schwarzmaier, Thomas; Kuusk, Joel; Ansko, Ilmar; Vabson, Viktor; Lau, Ian C.; Maclellan, Christopher; Marty, Sabine; Ondrusek, Michael; Servantes, William; Taylor, Sarah; Van Dommelen, Ronnie; Barnard, Andrew; Vellucci, Vincenzo; Banks, Andrew C.; Fox, Nigel; Vendt, Riho; Donlon, Craig; Casal, Tânia.
We present the results from Verification of Reference Irradiance and Radiance Sources Laboratory Calibration Experiment Campaign. Ten international laboratories took part in the measurements. The spectral irradiance comparison included the measurements of the 1000 W tungsten halogen filament lamps in the spectral range of 350 nm–900 nm in the pilot laboratory. The radiance comparison took a form of round robin where each participant in turn received two transfer radiometers and did the radiance calibration in their own laboratory. The transfer radiometers have seven spectral bands covering the wavelength range from 400 nm–700 nm. The irradiance comparison results showed an agreement between all lamps within ±1.5%. The radiance comparison results presented...
Tipo: Text Palavras-chave: Ocean colour; Spectral irradiance comparison; Spectral radiance sources comparison; Fiducial reference measurements.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00655/76688/77827.pdf
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Southern Ocean deep-water carbon export enhanced by natural iron fertilization ArchiMer
Pollard, Raymond T.; Salter, Ian; Sanders, Richard J.; Lucas, Mike I.; Moore, C. Mark; Mills, Rachel A.; Statham, Peter J.; Allen, John T.; Baker, Alex R.; Bakker, Dorothee C. E.; Charette, Matthew A.; Fielding, Sophie; Fones, Gary R.; French, Megan; Hickman, Anna E.; Holland, Ross J.; Hughes, J. Alan; Jickells, Timothy D.; Lampitt, Richard S.; Morris, Paul J.; Nedelec, Florence; Nielsdottir, Maria; Planquette, Helene; Popova, Ekaterina E.; Poulton, Alex J.; Read, Jane F.; Seeyave, Sophie; Smith, Tania; Stinchcombe, Mark; Taylor, Sarah; Thomalla, Sandy; Venables, Hugh J.; Williamson, Robert; Zubkov, Mike V..
The addition of iron to high- nutrient, low- chlorophyll regions induces phytoplankton blooms that take up carbon(1-3). Carbon export from the surface layer and, in particular, the ability of the ocean and sediments to sequester carbon for many years remains, however, poorly quantified(3). Here we report data from the CROZEX experiment(4) in the Southern Ocean, which was conducted to test the hypothesis that the observed north - south gradient in phytoplankton concentrations in the vicinity of the Crozet Islands is induced by natural iron fertilization that results in enhanced organic carbon flux to the deep ocean. We report annual particulate carbon fluxes out of the surface layer, at three kilometres below the ocean surface and to the ocean floor. We...
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Ano: 2009
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